Isolation of Actinomycetes from mangrove ecosystem in Torosiaje, Gorontalo, Indonesia

ABUBAKAR SIDIK KATILI♥, YULIANA RETNOWATI1Department of Biology, Faculty of Mathematics and Natural Sciences, Universitas Negeri Gorontalo. Jl. Jenderal Sudirman No. 6, Kota Gorontalo 96128, Indonesia. Tel: +62-435-821125-821752, Fax.: +62-435-821752, ♥email: dikykatili@gmail.com2Coastal Ecology Based Local Wisdom Research Center, Universitas Negeri Gorontalo. Kota Gorontalo 96128, IndonesiaManuscript received: 16 March 2016. Revision accepted: April 2017.

Abstract. Katili AS, Retnowati Y. 2017. Short Communication: Isolation of Actinomycetes from mangrove ecosystem in Torosiaje, Gorontalo, Indonesia. Biodiversitas 18: xxxx. Actinomycetes is a group of positive gram microorganism known to produce secondary metabolic compounds that works as antibiotics, anti-fungus, anti-virus, anti-cancer, and other important enzymes for the industry. This research aimed at discovering the potentials of Actinomycetes of mangrove ecosystem in Torosiaje, Gorontalo, Indonesia. It focused on finding out the diversity of Actinomycetes at mangrove ecosystem in Torosiaje. Hence, various types of Actinomycetes that have potentials to create secondary metabolic compounds/bioactive compounds for industrial purposes can be found. This purpose can only be attained through isolation of actinomycetes. This isolation was conducted through characterization of morphological characteristics of the colony and the spore, molecular characterization through isolation of DNA genome of the actinomycetes, amplication of 16S rRNA through PCR, sequencing and reconstruction of phylogenetic tree. We were able to obtain the actinomycetes isolate from the sediment in ryzosphere area of Ceriops tagal, Bruguiera gymnorrhiza, Xylocarpus sp., Rhizophora apiculata, Avichenia sp., and Sonneratia alba species of mangrove. Each isolated actinomycetes has specific morphological characteristics. The density of isolated actinomycetes in Torosiaje mangrove area was very low with an average of 1 x 105 CFU gram-1 in each sediment.Keywords: Actinomycetes, diversity, mangrove

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